Artificial intelligence-, organoid-, and organ-on-chip-powered models to improve pre-clinical animal testing of vaccines and immunotherapeutics: potential, progress, and challenges
Abstract
Vaccines and immunotherapies against infectious diseases and cancers have been a great success of the medical sciences over the last century. Pre-clinical testing in animal models has played a crucial role in the development of vaccines and immunotherapies, informing subsequent clinical trials. The current practices in pre-clinical animal model research must be approved by committees with strict policies and assessments on animal experiments including the “three Rs”: (1) Replacement, which assesses the scientific justification and rationale for using a live animal in biomedical research; (2) Reduction, which determines whether the number of animals required in an experiment is adequate to achieve scientifically valid results while reducing costs; and (3) Refinement, which ascertains that any given animal procedure will cause no to minimal pain or distress. The recent initiatives by the United States NIH and FDA to reduce or phase out animal testing in biomedical research underscore a growing interest in artificial Intelligence (AI), deep learning (DL), organoid, and organ-on-chip-powered models to slash the time and cost of preclinical animal research. This review highlights the strengths, progress, and limitations of these alternative pre-clinical research approaches, with a focus on vaccine and immunotherapeutic development. While the implementation of AI- and DL-, organoid-, and organ-on-chip-powered models will certainly help accelerate pre-clinical discoveries, modeling…
How strong is the evidence?
Evidence Hub reads this as a review, done in animal and cell cultures (in vitro), not in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health.
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