Evaluation of the Efficacy of an Avocado Oil-Based Lactobacillus rhamnosus Suspension in DNBS-Induced Colitis in Sprague-Dawley Rats
Abstract
Introduction: Inflammatory bowel disease (IBD) is a persistent inflammatory condition affecting the gastrointestinal tract (GIT), characterized by a spectrum of symptoms ranging from mild abdominal discomfort and diarrhoea to severe manifestations such as rectal bleeding, anemia, and, in extreme cases, fatal outcomes. Disruption of the gut microbiota, or dysbiosis, plays a key role in the onset and progression of IBD. Although corticosteroids remain a mainstay in treatment, their long-term use is associated with adverse effects, prompting interest in safer alternatives such as probiotics. Objectives: This study aimed to assess the therapeutic efficacy of a Lactobacillus rhamnosus suspension formulated in Avocado oil in a rat model of colitis induced by dinitrobenzene sulfonic acid (DNBS). Methods: Colitis was induced in Sprague-Dawley rats via intra-rectal administration of DNBS (120 mg/kg), followed by a 3-day induction period. Post-induction, animals were randomized into treatment groups receiving either oral dexamethasone (2 mg/kg/day) or L. rhamnosus suspension (3 × 10⁶ CFU/g/day) for 28 consecutive days. Evaluated parameters included changes in body weight, food and water intake, colon weight, histopathological alterations, colonic mucosal damage index (CMDI), and disease activity index (DAI). Biochemical markers analyzed comprised oxidative stress indicators (nitric oxide [NO], malondialdehyde [MDA]), antioxidant enzymes (glutathione [GSH], superoxide dismutase [SOD]),…
Read the paper
Related studies
- Prevalence and Characteristics of Autism Spectrum Disorder Among Children Aged 8 Years — Autism and Developmental Disabilities Monitoring Network, 11 Sites, United States, 2018
- Improving sleep quality leads to better mental health: A meta-analysis of randomised controlled trials
- Community transmission and viral load kinetics of the SARS-CoV-2 delta (B.1.617.2) variant in vaccinated and unvaccinated individuals in the UK: a prospective, longitudinal, cohort study
- Occupation and risk of severe COVID-19: prospective cohort study of 120,075 UK Biobank participants
- Clinical presentation and virological assessment of confirmed human monkeypox virus cases in Spain: a prospective observational cohort study
- Comparison of the characteristics, morbidity, and mortality of COVID-19 and seasonal influenza: a nationwide, population-based retrospective cohort study
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.