Homochiral Metal-organic Framework (+)-diacetyl-L-tartaric-NH-MIL-101(Fe) as Stationary Phase for Enantioseparation in Capillary Electrochromatography
Abstract
A novel enantioselective open-tubular capillary electrochromatography (CEC) column was fabricated by noncovalently immobilizing (+)-diacetyl-L-tartaric anhydride functionalized NH2-MIL-101(Fe) onto the capillary inner wall using polydopamine as an adhesive layer. The coating of the modified capillary was comprehensively characterized through Fourier-transform infrared spectroscopy, powder X-ray diffraction, Brunauer-Emmett-Teller analysis, scanning electron microscopy, energy dispersive X-ray spectroscopy, circular dichroism, and electroosmotic flow measurements. The column's chiral separation ability was assessed by analyzing seven model compounds, comprising four proton pump inhibitors (omeprazole, pantoprazole, lansoprazole, and tenatoprazole) and three fluoroquinolones (ofloxacin, sparfloxacin, and gatifloxacin). Under optimized conditions, baseline resolution (9.68-14.56) of all enantiomers was achieved within 7 min. The column demonstrated excellent reproducibility, with inter-day, intra-day, and column-to-column relative standard deviations ranging from 1.22% to 4.98%. Additionally, molecular docking simulations were conducted using AutoDock Tools to investigate interactions between the stationary phase and analyte enantiomers, with computational results corroborating experimental CEC data.
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