Comparing the Interactions of Trichomonas vaginalis/gallinae Legumain-Like Cysteine Protease 1 (LEGU-1) and Human Legumain (LGMN) Protein Sequences with Proton Pump Inhibitor Drugs (Lansoprazole, Omeprazole, and Esomeprazole) by Bioinformatics Analyses
Abstract
Purpose: The flagellar parasite Trichomonas vaginalis is the main cause of trichomoniasis cases globally and is associated with a broad range of complications. Due to the diverse range of virulence factors participating in the attachment, proliferation and resistance of this pathogen, preventive and well-tolerated compounds are necessary. One of the virulence factors in T. vaginalis, the legumain-like cysteine protease LEGU-1 is of particular interest as a target due to its potential influence on trichomoniasis and tumor development in urogenital systems, as well as its closely related to the avian strain T. gallinae. Previous studies on antineoplastic proton pump inhibitors revealed they also have legumain (LGMN) inhibitory activities.
Methods: Therefore, this study aimed to compare the molecular interactions of T. vaginalis/gallinae LEGU-1 and H. sapiens LGMN with proton pump inhibitor drugs (lansoprazole, omeprazole, and esomeprazole) through sequence analysis, 3D modeling, and molecular docking.
Results: Although sequence analyses revealed low homology between T. vaginalis/gallinae LEGU-1 and H. sapiens LGMN, secondary and 3D structural comparisons uncovered their structural conservation. Possible binding sites in all three proteins identified via CB-DOCK2 were compared to the previously described sites for LGMN, followed by targeted docking using Autodock Vina. Identification of amino acids mutually interacting with all three ligands by both programs revealed the…
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