Determination of 5-Hydroxylansoprazole/Lansoprazole Sulfone by HPLC-MS/MS in Incubation System in Vitro
Abstract
OBJECTIVE To establish a HPLC-MS/MS method for determing 5-hydroxylansoprazole/ lansoprazole sulfone,and optimize the incubated conditions for rat liver microsomes.METHODS 5-Hydroxylansoprazole/ lansoprazole were determined by HPLC-MS/MS.Single factor design was used to optimize the incubated conditions and the enzymes kinetics value was evaluated by graphical analysis with Lineweaver-Burk double reciprocal plots.RESULTS The linear range of 5-hydroxy lansoprazole and lansoprazole sulphone in liver microsome incubation system were 5.57-2 520 and 5.42-2 480 ng·mL-1,respectively.The optimal incubated conditions were 10 μmol·L-1 lansoprazole,0.16 mg liver microsomes,and 10min incubation,respectively.CONCLUSION The HPLC-MS/MS method is accurate and suitable for the determination of 5-hydroxy lansoprazole and lansoprazole sulfone in rat liver microsomes.The incubated condition can be applied for study in the drug interaction with lansoprazole.
Read the paper
Related studies
- The Prediction of the In Vitro Release Curves for PLGA-Based Drug Delivery Systems with Neural Networks
- Promising Ursolic Acid as a Novel Antituberculosis Agent: Current Progress and Challenges
- Proof of principle concept for the analysis and functional prediction of rare genetic variants in the CYP2C19 and CYP2D6 genes
- Fabrication, characterization, and docking studies of furosemide-loaded nanosponges using the emulsion solvent diffusion method
- The Effect of Concomitant Administration of Proton Pump Inhibitors on the Pharmacokinetics of CDK4/6 Inhibitors in Rats: Implications for the Evaluation of Hepatic and Transporter-Mediated Drug–Drug Interactions
- Mining the Metabolic Capacity of Clostridium sporogenes Aided by Machine Learning
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.