Ação de fármacos selecionados in silico sobre alvos funcionais em formas epimastigotas de Trypanosoma cruzi
Abstract
Chagas disease (CD) is an infection caused by the protozoan Trypanosoma cruzi, which is transmitted predominantly through contact with the waste of insect vectors known as "barbers". Although this disease represents a major challenge for public health, currently in Brazil there is only one drug for treating the disease, benznidazole, which has low efficacy in the chronic phase and severe adverse effects. The search for new drugs that can act more safely and efficiently throughout the infection is therefore justified. To this end, this study investigated potential functional targets such as replication, plasma membrane integrity, cell cycle, mitochondrial membrane potential, complexity and cell size of T. cruzi epimastigotes treated with the 50% Inhibitory Concentration (IC50) of the previously selected drugs in silico, oxiconazole nitrate and lansoprazole. This is an experimental study, carried out with in vitro assays, using a clonal population of Trypanosoma cruzi strain Dm28c, maintained under periodic repiques in Liver Infusion Tryptose (LIT) medium. The epimastigote form of the parasite was used for the experiments, which were subjected to treatment with different concentrations of drugs for 24 hours to determine the IC50/24h. For the flow cytometry tests, the parasites were treated with the IC50 of each drug for 24 hours. The effect of the drugs (IC50) on parasite replication was also assessed over 120 hours. Microsoft Excel 2020 and GraphPadPrism 7.04 were used to…
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Evidence Hub reads this as a thesis, done in computer models (in silico), not in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health.
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