Potencial atividade de fármacos combinados sobre formas epimastigotas de Trypanosoma cruzi
Abstract
Chagas disease, caused by the protozoan Trypanosoma cruzi, represents a major health risk in Latin America. Current treatments, benznidazole and nifurtimox, are limited by significant side effects and toxicity. To find better solutions, drug repositioning has become a promising approach. Recent research, based on in silico findings and subsequent tests, shows that oxiconazole nitrate and lansoprazole are effective in vitro against the epimastigote forms of T. cruzi Dm28c, sparking interest in exploring their combined effects, as well as their association with benznidazole. This study evaluated the in vitro effect of the combination of oxiconazole nitrate with lansoprazole and benznidazole on epimastigote forms of Trypanosoma cruzi. Experimental research using in vitro tests was conducted with a clonal population of the T. cruzi Dm28 strain. The study assessed the efficacy of the combinations by testing multiple proportions. Cell viability was measured after 72 hours of incubation with drug combinations using a colorimetric method with MTS and PMS. Based on the obtained results, the Combination Index and Dose Reduction Index were determined for the combinations. The cytotoxicity of the drug combinations on animal cells was determined through MTT assay, and the Selectivity Index of the combinations was calculated. In the viability assay with MTS, oxiconazole nitrate showed an IC50 of 91.1 μM, benznidazole had an IC50 of 23.5 μM, and lansoprazole showed no effect on cell…
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Evidence Hub reads this as a thesis, done in cell cultures (in vitro), not in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health.
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