Potencial atividade dos fármacos oxiconazol e lansoprazol sobre formas tripomastigotas de Trypanosoma cruzi Dm28c
Abstract
Chagas disease is caused by the protozoan Trypanosoma cruzi. It is classified as a neglected tropical disease and is endemic in Latin America, where up to 7 million people are believed to be infected. The available treatments for this disease are not very effective in the chronic phase and also have adverse effects. The process of discovering new drugs is long and expensive, so drug repositioning strategies are gaining ground as they can reduce costs and time to market. The aim of this study was to evaluate the activity of two drugs, previously selected in silico, on metacyclic trypomastigotes of T. cruzi Dm28c. Cytotoxicity tests were performed with the drugs oxiconazole (OXI) and lansoprazole (LZP) against Vero cells and metacyclic trypomastigote forms obtained in vitro, under chemically defined conditions, with the aim of determining the concentration capable of inhibiting 50% of parasite viability. Subsequently, tests were performed to evaluate the influence of the drugs on the process of metacyclogenesis in vitro. Using the CC50 (50% cytotoxic concentration) and IC50 (50% inhibitory concentration) data, the SI (selectivity index) was calculated. OXI showed a cytotoxic effect with a CC50 of 45.11 μM, in contrast to LZP which showed no cytotoxicity. OXI had an effect on trypomastigote forms with an IC50 of 279.6 μM and an IS of 0.16, while LZP had an IS of 2.46 and less activity against the parasite. Both drugs had an effect on the differentiation process, where…
Read the paper
Related studies
- The Prediction of the In Vitro Release Curves for PLGA-Based Drug Delivery Systems with Neural Networks
- Promising Ursolic Acid as a Novel Antituberculosis Agent: Current Progress and Challenges
- Proof of principle concept for the analysis and functional prediction of rare genetic variants in the CYP2C19 and CYP2D6 genes
- Fabrication, characterization, and docking studies of furosemide-loaded nanosponges using the emulsion solvent diffusion method
- The Effect of Concomitant Administration of Proton Pump Inhibitors on the Pharmacokinetics of CDK4/6 Inhibitors in Rats: Implications for the Evaluation of Hepatic and Transporter-Mediated Drug–Drug Interactions
- Mining the Metabolic Capacity of Clostridium sporogenes Aided by Machine Learning
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.