A Novel RP-UPLC Method for Elution and Quantification of Domperidone and Lansoprazole in Capsules: a Chemometric Optimization Approach
Abstract
Abstract The increasing prevalence of multi-component pharmaceutical formulations to enhance therapeutic effectiveness creates challenges in analysis procedures. These challenges require developing new, fast, reliable, and cost-effective methods for simultaneous drug quantification. In this study, a new reversed-phase ultra-performance liquid chromatography (RP-UPLC) method was developed for the simultaneous elution and quantification of domperidone (DMP) and lansoprazole (LSP) in a binary solid dosage form. To improve the efficiency and selectivity of the method, a chemometric optimization strategy based on a full factorial design (3³) was applied during the method development stage. This approach allowed accurate adjustment of key chromatographic variables, resulting in high resolution and significantly reduced analysis time. The optimized method achieved complete separation of LSP and DMP with retention times of 0.61 and 1.01 min, respectively, within a total runtime of just 2.0 min. Quantification was carried out at 283.0 nm using linear regression models based on the relationship between peak area and concentration. The method was validated and successfully applied to commercial capsule samples, showing its suitability for routine quality control. Overall, integrating chemometric design into UPLC method development proved to be a faster and more economical alternative to traditional methods for multi-component pharmaceutical analysis.
Read the paper
Related studies
- The Prediction of the In Vitro Release Curves for PLGA-Based Drug Delivery Systems with Neural Networks
- Promising Ursolic Acid as a Novel Antituberculosis Agent: Current Progress and Challenges
- Proof of principle concept for the analysis and functional prediction of rare genetic variants in the CYP2C19 and CYP2D6 genes
- Fabrication, characterization, and docking studies of furosemide-loaded nanosponges using the emulsion solvent diffusion method
- The Effect of Concomitant Administration of Proton Pump Inhibitors on the Pharmacokinetics of CDK4/6 Inhibitors in Rats: Implications for the Evaluation of Hepatic and Transporter-Mediated Drug–Drug Interactions
- Mining the Metabolic Capacity of Clostridium sporogenes Aided by Machine Learning
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.