The Effect of Pantoprazole, a Proton Pump Inhibitor, on Wound Healing in L929 Fibroblast Cells
Abstract
Aim: This study aimed to investigate the potential effects of pantoprazole on wound healing using the L929 fibroblast cell line. Specifically, its impact on cell viability, oxidative stress markers, and the in vitro wound healing process was evaluated.Material and Method: L929 fibroblast cells were treated with pantoprazole at concentrations between 2.5 and 40 μM for 24 hours to assess cell viability. To evaluate oxidative balance, 2.5 μM pantoprazole was applied, and total antioxidant status (TAS) and total oxidant status (TOS) were measured. For wound healing analysis, a scratch assay was performed by creating a standardized wound area (0.9 × 1.8 mm), and cell migration toward the wound was evaluated at 24-, 36-, and 48-hours post-treatment.Results: Pantoprazole showed a concentration-dependent cytotoxic effect at doses between 5 and 40 μM (p 0.05). Wound closure was significantly improved at 48 hours in the pantoprazole-treated group (p < 0.05), though earlier time points showed no statistical difference.Conclusion: At low concentrations, pantoprazole enhances fibroblast viability, boosts antioxidant status, and accelerates wound healing, indicating potential therapeutic utility in tissue repair and oxidative stress modulation.
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