Label-free four-dimensional live imaging of immune–stromal interactions in human alveolar tissue using higher harmonic generation microscopy
Abstract
Extract In order to improve clinical care for chronic lung diseases, particularly personalised therapies, model systems that capture the complex cellular ecosystem of lung tissue, where diverse immune cell populations interact with stromal cells and targets such as cancer cells, viruses, or bacteria within the alveolar architecture [1, 2], are eagerly awaited. Human tissue-based approaches such as precision-cut lung slices (PCLS) from surgically resected tumour lesions retain key features of the lung tissue microenvironment, including its architecture, mechanical properties, and diverse resident cell populations [3–5] making them suitable to test novel therapeutic agents [2, 6–8]. Further development of such technologies may contribute to the advancement of understanding the underlying disease mechanisms and improving therapeutic options for the treatment of chronic lung disease patients. Shareable abstract An innovative label-free time-lapse 4D higher harmonic generation microscopy with cultured 3D lung tissue slices will permit study of drug development and patient-specific treatment response prediction in the context of human lung disease. https://bit.ly/4gieKWK
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