Genotypic identification and antibiotic susceptibility of coagulase negative staphylococci isolates from urine samples of patients in southwest Nigeria
Abstract
Background: Urinary tract infection (UTI) largely affects women and is mostly caused by bacteria, among which coagulase negative staphylococci has received little attention. The objectives of this study are to isolate and genotypically identify coagulase negative staphylococci (CoNS) from urine samples of adult females attending selected healthcare facilities from three States (Oyo, Osun and Ogun) in southwest Nigeria, and to determine their susceptibility to selected antibiotics.Methodology: A case-control study of 471 adult female participants with urinary symptoms in 205 (case) and no urinary symptoms in 266 (control), was conducted over a period of 10 months. Mid-stream voided urine samples were collected from the participants. The samples were cultured on mannitol salt and blood agar plates, and bacteria growths were presumptively identified for CoNS and other bacterial isolates using routine biochemical tests. The CoNS isolates were confirmed by conventional PCR amplification with Staphylococcus saprophyticus specific primers and 16S rRNA sequencing with phylogenetic analysis using MegaX software 18 version 10.0.5. Antibiotic susceptibility testing was performed on genotypically identified CoNS isolates using VITEK 2 system. Chi square and Fisher Exact tests were used to compare the prevalence of bacterial UTIs across the States and between symptomatic and asymptomatic participants at α=0.05. Results: Of the urine samples of 471 participants examined, 178 (37.8%) had…
How strong is the evidence?
Evidence Hub reads this as a case-control, done in people. Its evidence level is 4 of 5 (Low · observational). Observational designs can show an association but not prove cause. Reported size: 471 participants or records.
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