GyrA Mutations in Nosocomial Ciprofloxacin-Resistant Escherichia coli Isolates Associated with Urinary Tract Infections
Abstract
Escherichia coli (E. coli) is one of most common organisms responsible for health care-associated urinary tract infection (HAUTI). The aim of this study was to determine antibiotic susceptibility pattern of E. coli isolated from HAUT I and to study ciprofloxacin resistance caused by gyrA gene mutations among these isolates. Urine Samples were collected from patients with suspected HAUTI. E. coli identification and antimicrobial susceptibility testing, minimum inhibitory concentration (MIC) of ciprofloxacin were done followed by detection of mutations in the gyrA gene by PCR-RFLP. Eighty seven isolates E. coli were isolated from HAUTI. Thirty-six isolates (41.3%) isolates were ciprofloxacin resistant. Double mutations at gyrA were detected at positions 83 and 87 of the quinolone resistance determining regions (QRDRs) in 25 (69.4%) ciprofloxacin resistant isolates, while single mutation at positions 87 revealed in 9 (25%) isolates. The pressure of the abuse of ciprofloxacin shares significantly to their resistance among E. coli isolated from urinary tract with double mutations at gyrA at positions 83 and 87 of the QRDRs represents an important factor for resistance.
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