Comparative genomic and phenotypic analysis of Escherichia coli ST1193 and ST131 from urinary and bloodstream infections: insights into resistance, virulence, and divergent strategies
Abstract
BACKGROUND: The global dissemination of multidrug-resistant (MDR) Escherichia coli (E. coli) clones, particularly the pandemic ST131 and the emerging ST1193, poses a serious public health threat. While ST131 is well-studied, the factors enabling ST1193’s rapid dissemination across both urinary and bloodstream infection niches remain unclear. METHODS: We compared 48 urinary (UPEC) and bloodstream (BPEC) E. coli isolates of ST1193 (n = 24) and ST131 (n = 24) from Shanghai, using integrated phenotypic-genotypic analysis, including antimicrobial susceptibility, whole-genome sequencing (WGS), hemolysin assays, Galleria mellonella larval infection model. RESULTS: Both lineages exhibited high rates of multidrug resistance with strongly genotype-phenotype concordance. Key contrasts emerged: ST1193-BPEC showed significantly higher resistance to trimethoprim/sulfamethoxazole than ST131-BPEC (p < 0.05), while ST131 overall demonstrated greater resistance to cefotaxime/cefazolin and a higher ESBL prevalence (ST131 83.33% vs. ST1193 50.00%, p < 0.05). All ST1193 isolates were resistant to fluoroquinolones but remained susceptible to carbapenems and ceftazidime-avibactam. Genomic analysis revealed striking conservation in ST1193, with near-uniform presence of fimH64 (23/24), K1 capsule (100%), and O75:H5 serotype (91.7%), along with unique virulence genes (vat, neuABCD, hcp2, kpsT, espL1). In contrast, ST131 displayed heterogeneity in fimH alleles (predominantly fimH30), K5 capsule…
Read the paper
Related studies
- Association of lower urinary tract symptoms and OAB severity with quality of life and mental health in China, Taiwan and South Korea: results from a cross-sectional, population-based study
- Ten-Day Quadruple Therapy Comprising Low-Dose Rabeprazole, Bismuth, Amoxicillin, and Tetracycline Is an Effective and Safe First-Line Treatment for Helicobacter pylori Infection in a Population with High Antibiotic Resistance: a Prospective, Multicenter, Randomized, Parallel-Controlled Clinical Trial in China
- The Role of the NLR and PLR in Urinary Tract Infection
- Threat of Antimicrobial Resistance among Pilgrims with Infectious Diseases during Hajj: Lessons Learnt from COVID-19 Pandemic
- Leclercia adecarboxylata urinary tract infection in a patient with bladder cancer and recurrent hematuria
- A Case Series of Staphylococcus lugdunensis Infection in Cancer Patients at an Academic Cancer Institute in the United States
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.