Identification, antifungal resistance, and genomic characterization of a single Candida auris isolate from urinary tract infection
Abstract
Objective To analyze the identification, antifungal resistance, and genomic characteristics of a Candida auris (C. auris) strain isolated from a urine specimen of an ICU patient at Yijishan Hospital, Wannan Medical College, Anhui Province. Methods The isolate was identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). The susceptibility of the isolates to fungi was determined by measuring the Minimum Inhibitory Concentration (MIC) values using the VITEK 2 COMPACT system. Whole-genome sequencing (WGS) was performed using high-throughput technology. Resistance and virulence genes were annotated using public databases, including NCBI (https://www.ncbi.nlm.nih.gov/, version 2.2.28), DFVF (http://sysbio.unl.edu/DFVF/, version 1.0), PHI-base (http://www.phi-base.org, version 5.0) and KEGG (https://www.kegg.jp/, version 89.1). A phylogenetic tree was constructed through analysis of the 18S rRNA nucleotide sequence. Results The isolate named CAS20503 was identified as C. auris. Antifungal susceptibility testing showed resistance to fluconazole and amphotericin B. Genomic analysis identified resistance genes including ERG11 (azole), FKS1 (echinocandin), ERG3 (polyene), and efflux pumps CDR1, MDR1. Resistance mutations were detected. The virulence genes analyzed based on the DFVF database included CaNik1, CHS2, DUR1,2, HSP90, ICL1, PMT1, PMT2, PMT4, SSD1, TPS2. The host pathogenic genes identified by comparison with the PHI-base…
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