Prevalence, Demographic Distribution, and Antimicrobial Resistance Patterns of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in Urinary Tract Infections: A Cross-Sectional Study in a Tertiary Care Hospital in Manipur, India
Abstract
Background: Urinary tract infections (UTIs) are among the most common bacterial infections globally, with Escherichia coli as the predominant uropathogen. The emergence of extended-spectrum beta-lactamase (ESBL)-producing strains has significantly complicated treatment due to widespread multidrug resistance (MDR). Objectives: This cross-sectional study aimed to determine the prevalence of ESBL-producing E. coli in UTIs, describe its demographic distribution, and evaluate antimicrobial susceptibility patterns among isolates from patients at a tertiary care hospital in Imphal, Manipur, India. Methods: From September 2015 to August 2017, 230 non-repetitive E. coli isolates from urine samples of symptomatic patients were collected, and antimicrobial susceptibility testing was done by Kirby-Bauer disk diffusion methodology. Isolates were then screened for ESBL production using Clinical and Laboratory Standards Institute (CLSI) guidelines. Screening employed disk diffusion with indicator cephalosporins, and confirmation for ESBL production was done via the combined disk test (CDT) (ceftazidime ± clavulanic acid). Results: Of the 230 isolates, 171 (74.3%) were screened positive and 97 (56.7%) were confirmed as ESBL producers by CDT. Female patients predominated with 185 (80.4%) cases; the male-to-female ratio was 1:4.1, with the highest prevalence in the 16-25 years age group, accounting for 44 (19.1%) cases. Community-acquired isolates represented 172 (74.8%) cases, with 68…
How strong is the evidence?
Evidence Hub reads this as a cross-sectional, done in people. Its evidence level is 4 of 5 (Low · observational). Observational designs can show an association but not prove cause.
Read the paper
Related studies
- Horizontal gene transfer in the human gastrointestinal tract: potential spread of antibiotic resistance genes
- Mutation supply and relative fitness shape the genotypes of ciprofloxacin-resistant Escherichia coli
- Genomic Survey of E. coli From the Bladders of Women With and Without Lower Urinary Tract Symptoms
- Characterization of cefotaxime-resistant urinary Escherichia coli from primary care in South-West England 2017–18
- Fitness cost constrains the spectrum of marR mutations in ciprofloxacin-resistant Escherichia coli
- Emerging evidence-based innovative approaches to control catheter-associated urinary tract infection: a review
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.