Phenotypic and Genetic Detection of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Klebsiella pneumoniae Isolates from Urinary Tract Infection Cases in Keffi, Nigeria
Abstract
Background: Multidrug-resistant Klebsiella pneumoniae are often associated with urinary tract infections in the community or hospital settings globally. Resistance resulting from the expression of the Extended-Spectrum Beta-Lactamase (ESBL) gene has been widely reported. In Nigeria, the prevalence has been reported to be between 16% to 62%.
Aim: The current study focused on the isolation of K. pneumoniae associated with urinary tract infections (UTIs) using standard microbiological methods, as well as the antibiotic susceptibility profile and the detection of Extended-Spectrum Beta-Lactamase (ESBL) genes using phenotypic and genetic procedures.
Study Design: The study was a cross-sectional study utilising random urine samples from patients with UTI in the selected hospitals.
Place and Duration of Study: The study was performed in Keffi from July to October 2024. Keffi is a local government area of Nasarawa State, Nigeria.
Methodology: Early morning midstream urine samples were randomly collected from 160 consenting participants from the six selected hospitals. The samples were cultured following standard microbiological techniques. Antibiotic susceptibility testing was performed using the Kirby-Bauer disk diffusion. ESBL producers were detected using the disk diffusion and PCR methods. Results obtained in this study were analysed using Smith’s Statistical Package (Version 2.8, California, USA) and a P value of ≤ 0.05 was considered statistically significant. The collected…
How strong is the evidence?
Evidence Hub reads this as a cross-sectional, done in people. Its evidence level is 4 of 5 (Low · observational). Observational designs can show an association but not prove cause. Reported size: 160 participants or records.
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