Genomic tracing and resolution of environmental Klebsiella pneumoniae ST307 CTX-M-15 linked to a nosocomial urinary tract infection in a cat: a veterinary teaching hospital case study
Abstract
BACKGROUND: Hospital-acquired infections (HAIs) are a significant concern in human medicine and have increasingly been reported in veterinary settings as well. Veterinary Teaching Hospitals may present a heightened risk for HAIs, partly due to the lack of standardized infection control measures and screening protocols. In May 2022, a cat previously hospitalized at a Veterinary Teaching Hospital for urinary tract obstruction developed a catheter-associated urinary tract infection (CAUTI). A multidrug-resistant (MDR) strain of Klebsiella pneumoniae was isolated. To investigate the suspected HAI and assess the presence of other MDR Gram-negative bacteria, an environmental screening of different hospital surfaces was conducted using both phenotypic and genotypic analyses. RESULTS: A total of 70 swabs were collected from environmental surfaces, 51 before and 19 after cleaning procedures. Sterility was observed in 19.6% (10/51) of samples collected before cleaning and in 26.3% (5/19) of those collected afterward. Four Klebsiella pneumoniae strains were isolated: two from the environment (a keyboard in the ultrasound room and a cattery cage) and two from the patient (urine and a bladder clot). All four isolates were identified as a single clone of K. pneumoniae sequence type ST307, carrying the CTX-M-15 gene, the GyrA-83I and ParC-80I point mutations, and the oqxA and oqxB resistance genes, which confer resistance to disinfectants. Environmental screening also yielded 13…
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Evidence Hub reads this as a case report, done in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health.
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