Bioactivity-Guided Isolation of Flavone Glycoside from Terminalia catappa: Evaluating Anti-MRSA and Anti-Dermatophytic Potential
Abstract
Antibiotic resistance is one of the major threats to public health in the twenty-first century. In this line of work, plants represent a priceless source of antimicrobial compounds since they house chemically different metabolites with a wide range of therapeutic applications. This study reports the bioactivity-guided fractionation, characterization, and evaluation of the efficacy of antimicrobial compounds from leaf acetone extracts of the traditional medicinal plant Terminalia catappa against bacterial clinical isolates and dermatophytes. The acetone extract of T. catappa was subjected to column chromatography for the separation and purification of the phytocompounds. The fractions were analyzed using a thin-layer chromatography-bioautography assay to detect the antimicrobial potency of the eluted compounds. The efficacy of the antimicrobial compounds was evaluated by the minimum inhibitory concentration, minimum bactericidal concentration, and minimum fungicidal concentration. Spectral characterization and structure elucidation of the compound were also achieved. The leaf acetone extract, when subjected to gradient elution by column chromatography, resulted in eight fractions. The fraction Fr-2 was subjected to thin-layer chromatographic elution, which resulted in the elution of phytocompound with Rf value of 0.50 and the phytocompound exhibited antimicrobial activity in the TLC-bioautography assay, and it was isolated in pure form and confirmed as Apigenin…
Read the paper
Related studies
- Horizontal gene transfer in the human gastrointestinal tract: potential spread of antibiotic resistance genes
- Mutation supply and relative fitness shape the genotypes of ciprofloxacin-resistant Escherichia coli
- Genomic Survey of E. coli From the Bladders of Women With and Without Lower Urinary Tract Symptoms
- Characterization of cefotaxime-resistant urinary Escherichia coli from primary care in South-West England 2017–18
- Fitness cost constrains the spectrum of marR mutations in ciprofloxacin-resistant Escherichia coli
- Emerging evidence-based innovative approaches to control catheter-associated urinary tract infection: a review
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.