Isolation and identification of bacteriophage against Escherichia coli ATCC 25922 and their biofilm Inhibition studies
Abstract
Bacterial biofilm development plays a crucial role in causing serious health concerns in the medical sector. The adhesion of germs on living and non-living surfaces can lead to damage as well as diseases in humans, and other animals. To address this problem, researchers focus exclusively on bacteriophage therapy. In the present study, lytic phage with 1010 PFU/mL was isolated from hospital sewage samples by targeting Escherichia coli (ATCC 25922). The morphological study of the isolated phage was examined under high-resolution transmission electron microscopy (HR-TEM) by using 2% of uranyl acetate as a negative staining, which revealed that the isolated phage belonged to the Myoviridae family. Bacterial turbidity assay showed reduced optical density (OD) in 0.01 multiplicity of infection (MOI) and no growth in 0.1 and 1.0 MOI phage concentration which was compared with control (without phage-treated E. coli cells). The phage-host interaction was investigated using an HR-TEM after treating the phage for 30 min, which showed the lytic cycle of the phage. Further, the E. coli biofilm was assessed using a microplate reader after 24 and 48 h on 96 titer plates with crystal violet staining to confirm the inhibition efficiency of phage-treated biofilm quantitatively (83%). Then the compound and fluorescence microscopy were used for qualitative measurement of inhibition activity. Further, the biofilm formation of E. coli on a coverslip surface was analysed using scanning electron…
Read the paper
Related studies
- Horizontal gene transfer in the human gastrointestinal tract: potential spread of antibiotic resistance genes
- Mutation supply and relative fitness shape the genotypes of ciprofloxacin-resistant Escherichia coli
- Genomic Survey of E. coli From the Bladders of Women With and Without Lower Urinary Tract Symptoms
- Characterization of cefotaxime-resistant urinary Escherichia coli from primary care in South-West England 2017–18
- Fitness cost constrains the spectrum of marR mutations in ciprofloxacin-resistant Escherichia coli
- Emerging evidence-based innovative approaches to control catheter-associated urinary tract infection: a review
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.