Resistant Pseudomonas aeruginosa carrying virulence genes in hospitalized patients with urinary tract infection from Sanandaj, west of Iran
Abstract
Abstract Background and objective Pseudomonas aeruginosa strains are important causes of urinary tract infection (UTI) in hospitals. The aims were to determine antibiotic susceptibility and presence of resistance and virulence genes in P. aeruginosa isolated from hospitalized patients with symptoms of UTI. Method A total of 50 P. aeruginosa were collected during January to June 2017 from hospitalized patients with symptoms of UTI in Sanandaj, west of Iran. Isolates were examined for susceptibility to 9 antibiotics using the disk diffusion method. Virulence genes and classes 1–3 integrons were detected by polymerase chain reaction. Cross resistance was determined in ciprofloxacin-resistant isolates. Results The most and least effective antibiotics were meropenem and ciprofloxacin (92% and 42% sensitivity, respectively). The ciprofloxacin -resistant isolates were significantly resistant to gentamicin (86.2%), aztreonam (62.1%), cefepime (55.2%), ceftazidime (41.4%), and amikacin (31%). The plcH hemolytic phospholipase C, toxA encoding exotoxin A, alginate algD, and class 1 integron were found in 98%, 94%, 6%, and 74% of the isolates, respectively. A relatively high percentage of the virulent isolates were multidrug resistant. Conclusions A high prevalence of plcH and toxA virulence factors was detected in resistant P. aeruginosa uroisolates from hospitalized patients. Integrons might be responsible for the dissemination of resistance genes. Our results highlight the need for…
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