Isolation and characterization of bacteriophages against antibiotic-resistance Pseudomonas aeruginosa
Abstract
Background and objectives: As an important opportunistic pathogen, Pseudomonas aeruginosa is associated with severe infections and increasing antimicrobial resistance. The growing prevalence of multidrug-resistant (MDR) strains has renewed interest in bacteriophages as alternative antibacterial agents. The present study sought to isolate and characterize lytic bacteriophages from hospital wastewater active against antibiotic-resistant clinical P. aeruginosa isolates.
Materials and methods: Fifty clinical isolates of P. aeruginosa were obtained and tested for antimicrobial susceptibility. Bacteriophages were isolated from hospital wastewater by enrichment and spot assay screening, followed by plaque purification and titration using the double-layer agar method. The host range of purified phages was evaluated against the clinical isolates. Adsorption assays and one-step growth experiments were performed to determine adsorption efficiency, latent period, and burst size. Transmission electron microscopy (TEM) was used to examine phage morphology.
Results: A considerable proportion of the clinical isolates showed multidrug-resistant phenotypes, and a considerable number were also classified as extensively drug-resistant. Four lytic bacteriophages, designated HSP1-HSP4, were successfully isolated and purified. All produced clear plaques and reached high titers. Host range analysis showed variable lytic spectra, with HSP1 exhibiting the broadest activity and showing lytic activity…
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