Extraction of Outer Membrane Proteins of Proteus Mirabilis Isolated From Urinary Tract Infections and their Immunological Effect In Vitro
Abstract
300 urine samples were collected from patients visiting Fallujah city hospitals (consulting clinics of Fallujah Teaching Hospital and Women and Children Hospital) for the period from 1/9/2020 to 30/12/2020, of different ages and for both sexes. The results showed that out of 300 urine samples were 244(81.33%) A sample with a positive result for bacterial culture, and it was found there were high significant differences between the positive and negative cultures. Females and males (64.70) (35.29), respectively. Isolates of Proteus mirabilis were diagnosed by observing the cultivar (colonies) and microscopic (bacterial cells) characteristics, biochemical tests and diagnostics with Vitek device. Antibiotic sensitivity were done at 12 types, Antibiotic resistance wre vary from Cefotaxime, Rifampicin, Ceftriaxon (100%), Cefixime, Ciprofloxacin (76.4%), Levofloxacin (64.7%), Nitrofurantoin (58.8%), Gentamicin (52.9%), Amikacin, Nalidixic acid (35.2%) (17.6%) Imipenem (11.7%). Extraction and partial purification of outer membrane proteins (OMPs) from the most antibiotic-resistant isolate using lysozyme, DNase, RNase, and N-Lauroyl-Sarcosinate enzymes. associated with the ion exchanger. use 25) of led to low significant of lymphocyte viability 97.66,98.0), respectively compared to With the negative control (99.66), the concentrations (100,50)% significantly reduced the percentage of survival of PMNs cells (97.66,98.33), respectively, compared with the control (99.66), while the…
Read the paper
Related studies
- Association of lower urinary tract symptoms and OAB severity with quality of life and mental health in China, Taiwan and South Korea: results from a cross-sectional, population-based study
- Ten-Day Quadruple Therapy Comprising Low-Dose Rabeprazole, Bismuth, Amoxicillin, and Tetracycline Is an Effective and Safe First-Line Treatment for Helicobacter pylori Infection in a Population with High Antibiotic Resistance: a Prospective, Multicenter, Randomized, Parallel-Controlled Clinical Trial in China
- The Role of the NLR and PLR in Urinary Tract Infection
- Threat of Antimicrobial Resistance among Pilgrims with Infectious Diseases during Hajj: Lessons Learnt from COVID-19 Pandemic
- Leclercia adecarboxylata urinary tract infection in a patient with bladder cancer and recurrent hematuria
- A Case Series of Staphylococcus lugdunensis Infection in Cancer Patients at an Academic Cancer Institute in the United States
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.