Efficacy of combination of nitrofurantoin with gentamicin, and ciprofloxacin against resistant E. coli isolated from patients with urinary tract infections: in vitro study
Abstract
The aim of this study to determined in vitro effects of various combinations of three antimicrobials (nitrofurantoin, gentamicin and ciprofloxacin) against twenty five E. coli isolates and the role of plasmidDNA in antimicrobial resistance. Method: Seventy five E. coli isolates were collected from urine of patients with urinary tract infections in AL-Kadhimia and AL-Yarmook teaching hospitals in Baghdad for a period between 22/11/2012 to 15/3/2013, from these samples twenty five isolates were selected according to their pattern of the highest resistance as these showing multidrug resistances and tested to specify their minimum inhibitory concentration for (nitrofurantoin, gentamicin and ciprofloxacin. The plasmid profile for the twenty five E. coli isolates were studied using Pure Yield ™ plasmid Miniprep system- Cat.# A1220 – Promega- USA. In order to determined the presence of plasmid for antimicrobials resistance. Result :Among combinations the combination of nitrofurantoin with gentamicin showed high synergistic effect when 1/4+1/4 MIC for each antimicrobial were used. While combinations of nitrofurantoin with gentamicin and ciprofloxacin in some isolates showed additive effect when 1/2+1/2 MIC for each antimicrobial were used. ), nitrofurantoin was found having the lowest MIC comparing with others.Extraction of plasmidDNA indicates the presence of antimicrobial resistance plasmid in (A6, A37, A32, and A57) isolates. Conclusion: Nitrofurantoin had more effect on E.coli…
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