Molecular Study of Some Virulence Genes of Escherichia coli Isolated from Women with Urinary Tract Infection in AL-Najaf City
Abstract
The current study aimed to investigate the presence of (shiA, sisA and sisB) genes in Escherichia coli bacteria which responsible virulence factors by using molecular technique (PCR). Out of 500 urine samples, preliminary results showed there was a bacterial growth in 327(65.4%) specimens. It was found that 232 specimens (46.4%) were diagnosed as gram negative bacteria, 91(27.82%) of it identified as E.coli by cultural, biochemical characteristics, API 20E System and Vitek-2 system. while there were 95 specimens (19%) were diagnosed as gram positive bacteria and 173 specimens (34.6%) with no any growth. The susceptibility test for 13 types of antibiotics drugs were tested by using disk diffusion method, the results demonstrated that isolates of E.coli showed resistance to, Amoxicillin+Clavulanic acid (92.3%) Trimethoprim–sulfamethoxazol and Cefotaxime (90.1%), Aztreonam (85.71%), Ceftazidime (76.92%), Ciprofloxacin (52.74%), Amikacin (50.54%), Ceftriaxone (48.35%), Gentamicin (42.85%), levofloxcin (26.37%), Nitrofurantoin (17.58%), Imipenem(7.69%), and Meropenem (4.39%). Showing clinical and laboratory signs of urinary tract infection (UTI).
Read the paper
Related studies
- Association of lower urinary tract symptoms and OAB severity with quality of life and mental health in China, Taiwan and South Korea: results from a cross-sectional, population-based study
- Ten-Day Quadruple Therapy Comprising Low-Dose Rabeprazole, Bismuth, Amoxicillin, and Tetracycline Is an Effective and Safe First-Line Treatment for Helicobacter pylori Infection in a Population with High Antibiotic Resistance: a Prospective, Multicenter, Randomized, Parallel-Controlled Clinical Trial in China
- The Role of the NLR and PLR in Urinary Tract Infection
- Threat of Antimicrobial Resistance among Pilgrims with Infectious Diseases during Hajj: Lessons Learnt from COVID-19 Pandemic
- Leclercia adecarboxylata urinary tract infection in a patient with bladder cancer and recurrent hematuria
- A Case Series of Staphylococcus lugdunensis Infection in Cancer Patients at an Academic Cancer Institute in the United States
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.