Genotyping Investigation of Ciprofloxacin Resistance Genes (Parc And Pare) In Clinical Pseudomonas Aeruginosa Isolates
Abstract
Abstract Purpose Due to Pseudomonas aeruginosa's low susceptibility to the development of antibiotic resistance and antimicrobial drugs, therapy and control of severe infections often result in recurrent difficulties. A bacterial isolate with multidrug resistance (MDR) is one that is resistant to at least one compound from more than three antimicrobial groups, such as aminoglycosides and fluoroquinolones (10). Human burn wounds are complex environments where necrotic tissue and exudates formed from plasma develop. Methods The samples were immediately cultivated on Pseudomonas aeruginosa-selective Blood, MacConkey, Nutrient, and Cetrimide agar media and incubated overnight at 37°C under aerobic conditions. Several biochemical tests, including Oxidase and Catalase tests, were used to identify P. aeruginosa. Additionally, the identification was confirmed using a quick identification procedure (VITEK 2 System). After adding 20% glycerol, the detected isolates were kept at -20°C. Results The findings of the local isolates' sequences being aligned with the reference strain revealed some mismatches in the nucleotides of our query at various locations, including deletions and replacements. According to the findings in table 4, there were discrepancies between the isolates from burn that were resistant to ciprofloxacin (P1 and P16) and the reference bacteria (CP034435). Conclusion The following are the findings of the current study: Pseudomonas aeruginosa is more prevalent in burn…
Read the paper
Related studies
- Horizontal gene transfer in the human gastrointestinal tract: potential spread of antibiotic resistance genes
- Mutation supply and relative fitness shape the genotypes of ciprofloxacin-resistant Escherichia coli
- Genomic Survey of E. coli From the Bladders of Women With and Without Lower Urinary Tract Symptoms
- Characterization of cefotaxime-resistant urinary Escherichia coli from primary care in South-West England 2017–18
- Fitness cost constrains the spectrum of marR mutations in ciprofloxacin-resistant Escherichia coli
- Emerging evidence-based innovative approaches to control catheter-associated urinary tract infection: a review
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.