Sleep enhancement mechanism of GABA-Producing lactic acid bacteria XBMU-SN-23 fermentation broth in a p-chlorophenylalanine-induced insomnia mouse model
Abstract
γ-Aminobutyric acid (GABA) is an important neurotransmitter found abundantly in animals, plants, and microorganisms. Lactobacilli recognized as natural GABA producers, have garnered significant attention due to their safety and functionality. In our study, we aimed to isolate a high GABA-producing Lactobacillus strain from traditional fermented foods and investigate its growth characteristics, acid-producing capacity, and stress tolerance. Subsequently, a p-chlorophenylalanine (PCPA)-induced insomnia mouse model was established to evaluate the sleep-enhancing effects and underlying mechanisms of the strain's fermentation broth. We isolated six strains of GABA-producing lactic acid bacteria, among which strain XBMU-SN-23 exhibited the highest GABA production (2.150 g/L) as determined by high-performance liquid chromatography (HPLC) and was identified as Lactiplantibacillus plantarum via 16S rDNA sequencing. Treatment with XBMU-SN-23 fermentation broth significantly improved the quiet state of PCPA-induced insomniac mice. It mitigated PCPA-induced damage to hypothalamic neuronal cells, increased the levels of GABA, serotonin (5-HT), interleukin-1 (IL-1), and melatonin (MT), while simultaneously reducing glutamate (Glu) and dopamine (DA) in the serum and hypothalamus of the mice, thereby contributing to the restoration of neurotransmitter balance. Additionally, the treatment led to an increase in the expression of 5-HT1A, GABAARα1, and GABAARγ2 receptor proteins, which play a…
Read the paper
Related studies
- Tryptophan Metabolism and Gut-Brain Homeostasis
- Rationale for a Multi-Factorial Approach for the Reversal of Cognitive Decline in Alzheimer’s Disease and MCI: A Review
- Prioritized Research for the Prevention, Treatment, and Reversal of Chronic Disease: Recommendations From the Lifestyle Medicine Research Summit
- Relationship between sleep disorders and gut dysbiosis: what affects what?
- The role of CLOCK gene in psychiatric disorders: Evidence from human and animal research
- Genetic, Transcriptome, Proteomic, and Epidemiological Evidence for Blood-Brain Barrier Disruption and Polymicrobial Brain Invasion as Determinant Factors in Alzheimer’s Disease
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.