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Effect of pearl millet diet on immunomodulating factors, physical performance and recovery in athletes: a randomised control trial

Saifi A, Kalpana K, Khanna GL, Benhur DR, Rakhra G, Tanwar E

Scientific reports, 2026

60 participants

Abstract

A pearlmilletbased diet may help modulate inflammatory cytokines and support better recovery in athletes exposed to high training stress. Its rich profile of fiber, minerals, and antioxidants suggests potential benefits for reducing inflammation and enhancing physical performance. However, evidence in athletic populations remains limited, prompting investigation into its functional impact. To explore the effect of pearl millet diet on immunomodulating factors, physical performance and recovery in athletes. A randomised controlled trial was conducted among male athletes aged 18-25 years (n = 60), who were randomly allocated into an experimental group (EG; n = 28) and a control group (CG; n = 30). The EG received a pearl millet-based diet (1/3 substitution of regular cereals with pearl millet) for 60 days, while the CG continued with their habitual cereal diet. Pre- and post-intervention assessments included body composition, immunomodulatory markers (IL-6, TNF-α, Hs-CRP), and measures of physical performance and recovery.Between-group differences at post-intervention were analysed using analysis of covariance (ANCOVA), adjusting for baseline values. Within-group changes were assessed using paired sample t-tests. Statistical significance was set at p < 0.05. The experimental group showed a significant increase in muscular strength (kg) from 35.3 ± 7.59 to 42.8 ± 9.14. VO₂ max (mL·kg⁻¹·min⁻¹) increased from 47.8 ± 4.76 to 49.5 ± 4.79, while resting heart rate (beats/min)…

How strong is the evidence?

Evidence Hub reads this as a randomized trial, done in people. Its evidence level is 2 of 5 (High · randomized trial). Randomized trials of a reasonable size are the strongest single-study design. Reported size: 60 participants or records.

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