Genomic characterization of extended-spectrum β-Lactamases (ESBLs)-producing Escherichia coli recovered from bloodstream infections in China
Abstract
BACKGROUND: Bloodstream infections (BSIs) caused by Escherichia coli represent a major clinical challenge, particularly among elderly patients and those with urinary catheters. The rising prevalence of extended-spectrum β-lactamase (ESBL)-producing strains further complicates therapeutic management and infection control. This study aimed to characterize the clinical features, antimicrobial resistance profiles, and genomic features of ESBL-producing E. coli isolates associated with BSIs in a county-level hospital in Zhejiang Province, China. METHODS: A total of 236 non-duplicate E. coli isolates recovered from BSIs were collected between January 2021 and December 2023. Antimicrobial susceptibility testing (AST) was performed for all isolates. Of these, 144 isolates were successfully preserved for subsequent molecular analysis, among which 65 ESBL-producing isolates underwent whole-genome sequencing (WGS) for the detection of ESBL genes, phylogenetic grouping, multilocus sequence typing, serotyping, fimH typing, and core genome SNP analysis. RESULTS: Most patients were elderly women, and urinary tract infection was identified as the primary source of bacteremia. Resistance rates were low to piperacillin-tazobactam (2.14%) and cefoperazone-sulbactam (1.28%) but high to levofloxacin (35.74%), ceftriaxone (47.01%), cefuroxime (50.21%), and trimethoprim-sulfamethoxazole (56.17%); all isolates remained fully susceptible to imipenem, ertapenem, amikacin, and tigecycline. Among 144…
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