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Isolation and Diagnosis of Antibiotic-Resistant Escherichia coli Bacteria from Urinary Tract Infection Patients in Mosul

Zainab Baqir Abbas, Hasan Faisal Hussein Kahya

Al-Kitab Journal for Pure Sciences, 2025

Abstract

Abstract In this study, 15 isolates of Escherichia coli were isolated from patients with urinary tract infections from Al-Khansa'a Teaching Hospital Mosul /Iraq from May 2023 till July 2023. The isolates were studied and diagnosed biochemically. The result of microscopic tests showed isolates that are short Gram-negative bacilli, and the result of biochemical tests was that the isolates were positive for catalase, triple sugar iron, indole, methyl red, coagulation enzyme, fermentation of the following sugars maltose, sucrose, galactose and glucose, while Escherichia coli isolates showed negative results for urease, Voges-Proskauer, and citrate tests. The isolates also showed resistance to all antibiotics used, with high resistance to both ampicillin, erythromycin, and cephalothin, which amounted to 100%. 60% for nalidixic, 53.33% for both azithromycin and levofloxacin and the lowest resistance rate shown by the isolates to gentamicin was 46.7%.

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