Molecular Detection of papE Virulence Genes in Escherichia coli Isolated from Women with Urinary Tract Infection
Abstract
Background: Urinary Tract Infections (UTIs) are among the most prevalent bacterial infections in women, often caused by uropathogenic Escherichia coli (UPEC). The papE gene, a component of the P fimbriae operon, is recognised as a key virulence factor facilitating bacterial adhesion and colonization of uroepithelial cells. Objective: This study aimed to detect the presence of the papE virulence gene in clinical E. coli isolates obtained from women with UTIs using molecular methods. Methods: A total of 116 urine samples from women with clinically diagnosed UTIs were collected. E. coli isolates were identified using standard microbiological methods and confirmed by the VITEK® 2 Compact system. Genomic DNA was extracted from the isolates and PCR amplification of the papE gene was performed using gene-specific primers. Amplification products were analysed by electrophoresis on 1.5% agarose gels. Results: According to Sex Distribution: Out of the 116 clinical bacterial isolates, 41 (36.6%) were from male patients, while 71 (63.4%) were from female patients. The isolates’ resistance to quinolone antibiotics, which includes nalidixic acid, ciprofloxacin and levofloxacin, was at 82.5, 56.3 and 50%, respectively. Cephalosporins, which include cefoxitin, cefotaxime, ceftazidime, cefepime and ceftriaxone, had a resistance of 53.3, 60, 76.6, 66.6 and 53.3%, respectively, for each aforementioned antibiotic. The isolates’ resistance to carbapenems, which includes meropenem and imipenem,…
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