rmtB Gene In Aminoglycoside Resistant Escherichia Coli From Patients With Urinary Tract Infection
Abstract
Introduction: Urinary Tract Infections (UTIs) are among the most common bacterial infections, ranging from mild to life-threatening cases. While antibiotics are the primary treatment, widespread use has led to the rise of multi-drug resistant (MDR) organisms. Objectives: This study aimed to assess the antibiotic susceptibility patterns of Escherichia coli isolated from urine samples, determine the minimum inhibitory concentration (MIC) of aminoglycoside drugs (amikacin and gentamicin), and detect the presence of the 16S rRNA methylase gene, rmtB, in aminoglycoside-resistant isolates. Methodology: A cross-sectional hospital-based study was conducted at Kathmandu Model Hospital from October to March 2022. A total of 1645 urine samples from UTI patients were processed using conventional culture methods. E. coli isolates were tested for antibiotic susceptibility using the modified Kirby Bauer disc diffusion method. MIC values for gentamicin and amikacin were determined using the agar dilution method, and conventional PCR was performed to detect the rmtB gene. Results: Of the 1645 samples, 305 (18.54%) exhibited significant bacteriuria, with E. coli accounting for 74.10% of cases. UTI was more prevalent in females (77.88%) than males (22.12%). Of the 226 E. coli isolates, 43.81% were MDR, and 11.06% were resistant to gentamicin. Among gentamicin-resistant isolates, 36% harbored the rmtB gene, all of which were also resistant to amikacin. The rmtB gene was associated with isolates…
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Evidence Hub reads this as a cross-sectional, done in people. Its evidence level is 4 of 5 (Low · observational). Observational designs can show an association but not prove cause.
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