Clinical and molecular study of upper respiratory tract infections of cats caused by Bordetella bronchiseptica in cats at Baghdad city
Abstract
This study included clinical and molecular study of Bordetella bronchiseptica from upper respiratory tract of 150cats with and without respiratory signs of (60 local breed and 90 different breed) and both sexes (70 male and 80female) with different ages including 85 cats more than one year and 65 less than one year private veterinaryclinics and Baghdad veterinary hospital in Baghdad city at the period from October 2023 to September2024.Totally, 150 nasal swabs were collected from cats at aseptic condition to carry out for bacteriology isolationby culturing Blood agar and Bordet Gengou agar base for culturing of B. bronchiseptica. Then the bacteria wereexamined by Gram stain and apply different biochemical and antimicrobial study using Vetik analysis of isolatedbacteria with confirmative diagnosis by molecular detection of FIM gene of B. bronchiseptica. The results ofclinical study showed that the most frequent signs were fever and dyspnea. The total isolation rate of B.bronchiseptica was 4.6%. The DNA of 7 isolates of B. bronchiseptica was successfully amplified by usingspecific primers at 425 bp of FIM gene of B. bronchiseptica.
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