Prevalence of Helicobacter Pylori Genotypes and Their Association With Upper Gastrointestinal Diseases: a Cross Sectional Study in Southern Iran
Abstract
Background: Investigating the prevalence of vacuolating cytotoxin (vacA), cytotoxin associated gene A (cagA), glm M genotypes, and subtypes of vacA of Helicobacter pylori (H. pylori) isolate in Jahrom, Southern Iran. DNA extracted from H. pylori samples retrieved from gastric biopsy isolated from 113 dyspeptic patients with positive rapid urease test (RUT). Genotyping was done by polymerase chain reaction (PCR) technique, using primers for vacA (s1a, s1b, s1c, s1, s2, m2, and m1), cagA, and glmM. Endoscopy was done for all the patients to screen upper gastrointestinal (GI) disorders. Results: GlmM was detected in 100% of the cases. VacA subtypes s1am2, s2m2, s1a, s1b, and s1c were detected in 27.9%, 25.6%, 50%, 3.5% and 2.4% of the isolates, respectively, while cagA was detected in 60.5% of the isolates. VacA alleles m1, s1, and s2 were detected in 54%, 50%, and 44% of isolates respectively. Also, 60.5% of the isolates were cagA-vacA-positive. A significant correlation was observed between vacAs1bm1 and gastroesophageal reflux disease (GERD) and glmM and normal esophagus. The presence of vacAs1bm1 and vacAs1bm2 has a significant association with gastric erythema. The presence of cagA showed a significant association with normal esophagus and hiatal hernia.Conclusions: In our research, the number of glmM and cagA positive isolates is higher among other genotypes and cagA is correlated with hiatal hernia, and normal esophageal finding is correlated with glmM genotype. There…
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