Ethanolic seed extract of Artocarpus heterophyllus ameliorates histomorphological, morphometric and functional alterations in the rat testis induced by sildenafil citrate
Abstract
Abstract Background: One in every ten men is affected by ED regardless of their age. Sildenafil citrate is the first-line drug in the treatment of ED and has been associated with seminiferous tubules and testicular degeneration, translating into decreased spermatogenesis. The study evaluated the effect of ethanolic seed extract of Artocarpus heterophyllus on changes in testicular histology, seminal fluid and serum testosterone levels alone and in ameliorating testicular changes induced by Sildenafil Citrate. Methods: Thirty-six adult male Wistar rats were randomly divided into six groups (n=6) and orally dosed daily with NS, seed extract of AH (500 or 1000 mg/kg), SC (50 mg/kg) or AH+SC (1000 mg/kg + 50 mg/kg or 1000 mg/kg + 100 mg/kg). Testicular histopathological and semen analyses were performed via H&E, PAS and Masson’s trichome staining and microscopy. Differences in sperm parameters, testicular size, seminiferous tubule characteristics, Leydig cell volume, and serum testosterone levels were determined. Results: SC administration significantly decreased the sperm count (3.47±1.46), motility (23.414±6.63) and morphology (0.038±0.02) compared to the negative control (6.04±0.47, 27.888±0.81 and 0.058±0.02, respectively). The administration of AH 1000 to SC-positive rats improved these parameters in an SC dose-dependent manner, i.e., 10.54±1.48, 31.516±0.99 and 0.0781±0.02 and 9.36±0.36, 27.314±1.45 and 0.049±0.03 for 50 mg/kg and 100 mg/kg SC, respectively. AH improved the…
How strong is the evidence?
Evidence Hub reads this as a preprint, done in animal, not in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health. Reported size: 6 participants or records.
Read the paper
Related studies
- Drug repositioning: a brief overview
- Cyclic nucleotide phosphodiesterases in Drosophila melanogaster
- Time-resolved in silico modeling of fine-tuned cAMP signaling in platelets: feedback loops, titrated phosphorylations and pharmacological modulation
- Current View on Green Tea Catechins Formulations, Their Interactions with Selected Drugs, and Prospective Applications for Various Health Conditions
- In silico Structure-based Identification of Novel Acetylcholinesterase Inhibitors Against Alzheimer’s Disease
- Development and In Vitro Evaluation of Controlled Release Viagra® Containing Poloxamer-188 Using Gastroplus™ PBPK Modeling Software for In Vivo Predictions and Pharmacokinetic Assessments
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.