Histopathological evaluation of the effects of sildenafil on organ damage in a diabetic rat model
Abstract
Aims: In this study, it was aimed to show the effects of sildenafil on heart, liver and kidney tissues histopathologically by creating an experimental diabetes model with streptozocin. Methods: Male adult Sprague dawley rat (48) was used in the study. The rats were first divided into three groups as control group, the diabetes group and the diabetes+sildenafil group. Each group was divided into two groups within itself. Streptozotocin 40 mg/kg was administered intraperitoneally to the rats in the groups that would develop diabetes and diabetes+sildenafil diabetes. Rats with blood glucose levels of 250 mg/dl and above after 48 hours were considered diabetic. Sildenafil citrate 10mg/kg/day was given by gavage to the diabetes+sildenafil group. At the end of the experiment heart, liver and kidney tissues were placed in formaldehyde solution. Hematoxylin-Eosin staining was applied to the sections taken. Histological changes in the stained sections were evaluated by a histologist. Histological evaluation was performed semi-quantitatively in heart, liver and kidney tissue. In the assessment, the findings of the tissues were scored and statistical analysis was performed. Results: Histological findings of heart, liver and kidney tissues were examined. It was determined that less organ damage was seen in the diabetes+sildenafil group compared to the Diabetes group. Conclusion: In our study, it has been demonstrated histologically that sildenafil can be a drug that has an antioxidant…
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