Boehmeria nivea (L.) Gaud. ameliorates airway inflammation by inhibiting Th2 cell differentiation in allergic asthma
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE
Boehmeria nivea (L.) Gaud. (BN) is a well-known traditional herbal medicine used to treat a wide range of conditions, including bleeding, fever, diarrhea, and wounds, as well as respiratory disorders such as cough and wheezing. However, its potential efficacy against allergic airway inflammation has yet to be systematically evaluated.
AIM OF THE STUDY
This study aimed to investigate the therapeutic potential of BN, particularly the ethyl acetate fraction (EA-BN), in modulating immune responses in allergic asthma.
MATERIAL AND METHODS
BALB/c mice were sensitized with ovalbumin (OVA) to induce allergic asthma and treated orally with BN fractions (100 mg/kg; EA-BN-L; 100 mg/kg and EA-BN-H; 200 mg/kg). Th2 cytokines (IL-4, IL-5, and IL-13) and inflammatory cell counts in bronchoalveolar lavage fluid (BALF) were measured. Bone marrow-derived dendritic cells (BMDCs) stimulated with lipopolysaccharide (LPS) and tumor necrosis factor (TNF)-α-stimulated NCI-H292 epithelial cells were used to assess in vitro potential effects. Flow cytometry and qPCR were used to evaluate cytokine levels and transcription factor expression.
RESULTS
This study demonstrated the significant efficacy of EA-BN in an OVA-induced allergic asthma model. EA-BN treatment markedly suppressed the expression of extracellular cytokines (IL-10, IL-12, and TNF-α), co-stimulatory molecules (CD80 and CD86), and MHC class I/II in both LPS-stimulated BMDCs and splenic DCs in OVA-induced…
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