Green synthesis of silver nanoparticles using Melia azedarach leaf extract and their antifungal activities: In vitro and in vivo
Abstract
Abstract The present work reported the green synthesis, characterization and antifungal activities of silver nanoparticles (AgNPs). The synthesis of AgNPs has been produced by mixing silver nitrate solution with aqueous Melia azedarach leaf extract as a reducing and stabilizing agent for AgNPs. After only 10 min of reaction, the light yellow color of the M. azedarach leaf extract was changed to brown due to the reduction of silver ions to AgNPs, under ambient conditions. The growth of biosynthesized AgNPs was monitored by UV–Visible spectroscopy complemented by dynamic light scattering technique (DLS), zeta potential, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and fourier transform infrared spectroscopy (FTIR). The peak absorbance of the UV–vis spectra was at 400 nm confirming the formation of silver nanoparticles. SEM analysis shows the existence of small spherical nanoparticles with a size ranged from 18 to 30 nm (average 23 nm). XRD analysis shows that the AgNPs were face-centered cubic (fcc) lattice. The EDS analysis displays intense signals of the silver element. FTIR analysis demonstrated that the hydrolyzable tannic acid involved in M. azedarach leaf extract responsible for the bioreduction and stabilization of AgNPs. The stability of AgNPs was confirmed by zeta potential analysis. A negative zeta potential value of −13.1 mV indicated the stability and good dispersion of AgNPs. The present report aims to…
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