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Evaluation of chemical composition of root canal dentin between two age groups using different irrigating solutions: An in vitro sem-eds study

Naresh Kumar K, Abhijith Kallu, L Surender, Sravani Nirmala, Narender Reddy

International Dental Journal of Student Research, 2024

Abstract

The study aimed to evaluate and compare the effect of different irrigating solutions on the chemical composition of root canal dentin between two age groups. Sixty-six extracted single rooted human premolar teeth were decoronated and longitudinally sectioned to expose canals. Root halves were divided into 2 groups and 3 sub-groups based on the irrigation protocol. Samples were evaluated for mineral content with Scanning Electron Microscope/Energy Dispersive Spectroscopy (SEM/EDS). The data were analyzed statistically. Results revealed a statistically significant difference in calcium levels of younger age group, with higher levels for SmearClear (19.1±1.31) when compared to MTAD (Mixture of Tetracycline Isomer, Acid and Detergent) (17.1±1.16), with p value 0.01. A statistically significant difference was observed in magnesium levels of younger age group, with higher levels for MTAD (0.95±0.21) when compared to SmearClear (0.82±0.22), with p value 0.05. Mean comparison of Ca/P ratio of younger age group, revealed higher values for SmearClear (1.71±0.14) when compared to MTAD (1.56±0.15), with p value 0.02. MTAD caused significantly greater loss of calcium, phosphorous, sodium and altered Ca/P ratio of root dentin among younger age group. On the other hand, loss of magnesium content was found to be higher within SmearClear sub group of younger age group (p=0.05).

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