Pigment Content and Gas Exchange of Red Raspberry In Vitro and Ex Vitro
Abstract
Abstract CO 2 uptake and various leaf parameters were examined including photosynthetic pigment content (chlorophyll a, b, and total carotenoids), fresh/dry weight, percentage of water content, gram dry weight/area, and total plantlet leaf areas of an aseptically cultured clone of red raspberry ( Rubus idaeus L.) incubated at 5 light intensities, from 2 to 6 klx. Cultured plantlets demonstrated relatively low levels of CO 2 uptake, averaging 2.5 mg CO 2 dm –2 hr –1 and rarely exceeding 4 mg CO 2 dm –2 hr –1 at saturating light intensities. Pigment content was higher in plantlets incubated at lower light intensities (2 to 4 klx). Cultures incubated at 3 klx were evaluated both at the time of transfer to soil and 1 month later. Plantlet leaves retained from culture could be distinguished from new leaves by tagging all plantlet leaves prior to soil transfer; both were assessed separately 1 month after transplantation. Leaves retained from culture, 30% of the total leaf area of transplants, contributed less than 10% of the CO 2 uptake at 3 klx. These leaves accounted for 10% to 30% of the total leaf area at higher light intensities but were net respirers. There was an increase in dry matter accumulation at 6 and 9 klx in these tagged leaves, but not at 3 klx. Continued accumulation of dry matter by the tagged leaves can be only at the expense of photosynthetic activity of the newly formed leaves. New leaves of transplants had a greater dry matter accumulation at 9 klx and a…
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