Arachidin 3 Modulation of Lipid Metabolism in Rotavirus Infections
Abstract
Rotavirus (RV) can cause severe and deadly gastroenteritis in young children, infants, and immunocompromised individuals. Previous studies have shown that arachidin 3 (A3) inhibits RV replication, and that RV replication is dependent on the presence of lipids. This study investigated the alteration of lipid metabolism by A3 in RV infected HT29.f8 cells. A decrease in the RV regulation of lipid biosynthesis genes was observed with the addition of A3 using qRT-PCR. Also, immunofluorescent and histochemical staining for neutral fats, a major component of cellular lipid droplets, revealed an increased accumulation with both RV and RV+A3 when compared to no virus and A3 controls. Furthermore, a western blot time course study of perilipin 1 presented a cycling pattern of expression with slight variations between RV, RV+A3, and A3. This data implies an association between A3 inhibition of RV replication and lipid metabolism that could be developed into a RV therapeutic treatment.
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