Effect of L-methionine on RBCs metabolism during storage
Abstract
Packed Red blood cells (RBCs) remain the most widely transfused blood component in the clinical setting. For clinical purposes, erythrocyte concentrate can be stored at 4 ± 2° C, for up to 42 days. However, their storage under blood banking conditions is associated with deleterious morphological and metabolic alterations, so called “storage lesions”, which have a negative effect on the quality of stored RBCs. Application of omics technology in transfusion medicine significantly improved the study of RBC storage lesions. Metabolomics analysis showed a dramatic decrease in methionine concentration in stored RBCs, while homocysteine level increased [1]. Methionine is an essential methyl donor for several metabolic reactions, such as regulation of glycolytic enzymes, synthesis of glutathione and conversion of phosphatidylethanolamine (PE) to phosphatidylcholine (PC). Methionine can be generated either by homocysteine and betaine, or by homocysteine and 5’-methyltetrahydrofolate (5’-MTHF). The latter reaction uses Cobalamin (vitamin B12) as a cofactor. Based on this finding, we attempted to restore the methyl-group pool in stored RBCs by supplementation of L-methionine, or to convert the accumulated homocysteine into methionine by supplementation of 5’-MTHF and vitamin B12. Study Design and Methods: A pool- and-spilt design of leukodepleted RBCs stored in PAGGS-M additive solution was carried out to obtain three identical groups for PAGGS-M (control), PAGGS-MM (supplemented with…
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