miR-143-3p integrates contractility and growth in benign prostate stromal cells
Abstract
BACKGROUND: Prostate smooth muscle contraction and stromal growth are key determinants of voiding symptoms in benign prostatic hyperplasia (BPH), but their regulation by microRNAs is unknown. Here, we assessed the functional role of miR-143-3p in contractility and growth-related functions in human prostate stromal cells. METHODS: Stromal (WPMY-1) and epithelial (BPH-1) cells were transfected with miR-143-3p mimic or inhibitor and analyzed using functional assays for contraction, proliferation, apoptosis, and viability. RESULTS: In WPMY-1 cells, miR-143-3p levels were strongly increased by mimic and markedly reduced by inhibitor transfection. In collagen matrix assays, contractions were enhanced by the mimic and reduced by the inhibitor. Similarly, agonist-induced contractions were attenuated by the inhibitor and slightly increased or enhanced by the mimic. These effects were accompanied by reduced calponin expression and reduced actin filaments following inhibitor transfection. The inhibitor further decreased proliferation and viability, reduced colony formation, and increased apoptosis, while the mimic exerted opposite or minor effects. In contrast, despite effective modulation of miR-143-3p expression, BPH-1 epithelial cells showed negligible functional responses. CONCLUSIONS: miR-143-3p promotes contractility and growth while suppressing apoptosis in prostate stromal cells, indicating a coordinated regulation of BPH-relevant functions. Targeting miR-143-3p may represent a…
Read the paper
Related studies
- Treatment of non-metastatic castration-resistant prostate cancer: facing age-related comorbidities and drug–drug interactions
- The Efficacy of Flogofilm® in the Treatment of Chronic Bacterial Prostatitis as an Adjuvant to Antibiotic Therapy: A Randomized Prospective Trial
- Inhibition of growth and contraction in human prostate stromal cells by silencing of NUAK1 and -2, and by the presumed NUAK inhibitors HTH01-015 and WZ4003
- Effectiveness and Safety of a New Nutrient Fixed Combination Containing Pollen Extract Plus Teupolioside, in the Management of LUTS in Patients with Benign Prostatic Hypertrophy: A Pilot Study
- Extract from Cucurbita pepo improves BPH symptoms without affecting sexual function: a 24-month noninterventional study
- Aquablation vs. holmium laser enucleation of the prostate for benign prostatic hyperplasia: a 150-patients prospective comparative multicenter study
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.