HPLC Analysis of nine corticosteroids in “natural creams” for atopic eczema
Abstract
Purpose: The aim of the study was to determine whether “natural creams” sold for treatment of childhood atopic eczema illegally contain corticosteroids with a newly developed rapid and simple HPLC analysis with UV detection. Material and Methods: HPLC analysis was performed using a Schimadzu LC-2010 chromatographic system (Schimadzu, Kyoto, Japan) consisting of a LC-20AT Prominence liquid chromatography pump with DGU-20A5 Prominence degasser, a SPD-M20A Prominence Diode Array Detector, and a SIL-20 AC Prominence auto sampler. Data analyses were done using Class VP 7.3 Software. The elution was carried out on a column Purospher STAR® RP 18e (250 x 4.6 mm i.d., particle size 5m), with a mobile phase consisted of acetonitrile and water in a gradient mode, at a flow rate of 1.0 mL min-1, at controlled column temperature (25oC). Detection of nine different corticosteroids (dexamethasone, prednisolone, methylprednisolone, fluocortolone, hydrocortisone, mometasone, betamethasone, beclomethasone, and diflucortolone) was carried out with DAD detector at a wavelength of 240 nm. The injection volume was 10 μl. The samples were five different creams sold for the treatment of childhood atopic eczema (marketed as steroid free) and were submitted by the state regulatory authority, Bureau of the medicines. Results: The method was fully validated according to the ICH (International Conference on Harmonization) guidelines by the determination of linearity, precision, and accuracy, limit of…
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