Molecular epidemiology and antibiotic resistance profiling of Staphylococcus aureus isolates from camel mastitis
Abstract
Mastitis is considered one of milk-producing animals' most widespread infectious diseases. The present study evaluated the prevalence of antibiotic-resistant isolates of Staphylococcus aureus (S. aureus) including methicillin-resistant S. aureus (MRSA), β-lactam-resistant S. aureus (BRSA), aminoglycoside-resistant S. aureus (ARSA), and tetracycline-resistant S. aureus (TRSA) from the udder of dromedary camels along with the associated risk factors and the antibiogram of resistant isolates. Phylogenetic analysis of antibiotic-resistant genes with NCBI sequences was performed to check their homology. A total of 384 milk samples were collected and subjected to standard microbiological procedures to isolate S. aureus. The results revealed that 177 milk samples were found positive for subclinical mastitis (SCM) out of which 101 milk samples were found positive for S. aureus. The molecular assay found the prevalence of MRSA, BRSA, ARSA, and TRSA as 48.51 %, 46.53 %, 42.57 %, and 39.60 % by targeting the mecA, blaZ, accA-aphD, and tetK genes respectively. The study isolates significant similarities to each other and to previously reported sequences from other countries that were found by in-silico analysis, indicating the possibility of pathogen transboundary transmission. This study also revealed potential risk factors that aid in the spread of mastitis in camels. Among various risk factors, the most significant were farm hygiene, physiological status of animals, type of mastitis,…
Read the paper
Related studies
- Ferulic acid regulates the AKT/GSK-3β/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model
- Novel Pharmacokinetic/Pharmacodynamic Parameters Quantify the Exposure–Effect Relationship of Levofloxacin against Fluoroquinolone-Resistant Escherichia coli
- Photochemical behavior of levofloxacin
- Correlation Between Dissolution Profiles of Salt-Form Drugs in Biorelevant Bicarbonate Buffer and Oral Drug Absorption: Importance of Dose/ Fluid Volume Ratio
- Evaluation of the synergistic potential and mechanisms of action for de novo designed cationic antimicrobial peptides
- Synthesis of [11C]levofloxacin
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.