Understanding the impact of moxifloxacin on immune function: Findings from cytokine analyses and immunological assays in mice
Abstract
(1) Background: Moxifloxacin (MXF) is a fluorinated quinolone antibiotic used most commonly due to its broad spectrum of activity. However, the immunomodulatory effects of MXF remain underexplored. This study aims to investigate the patterns of immunomodulatory effects. (2) Methods: Swiss female albino mice were treated with different concentrations of MXF, and the immunological studies were performed using a cytokine assay, carbon clearance test, indirect hemagglutination test, and a mice lethality assay. (3) Results: Results revealed that MXF mitigated cyclophosphamide-induced leukopenia, with TLC reduced less at 7.5 mg/kg (5.37 ± 0.6) compared to 3.75 mg/kg (2.95 ± 0.485) and 15 mg/kg (2.14 ± 0.104). Results of the phagocytic index showed significantly lower clearance rates in the 7.5 mg/kg (0.01600 ± 0.00175) and 15 mg/kg (0.01331 ± 0.00310) groups compared to the control. Ex-vivo mice macrophages cytokines analysis showed elevated TGF-β1 levels in the MXF 32 µg/ml group (30.826 ± 0.817pg/ml) and IL-10 levels in the MXF 16 µg/ml group (50.427 ± 0.786pg/ml). The MXF 64 µg/ml treated group showed significantly lower IL-6 (8.714 ± 0.647pg/ml) and TNF-α (28.81 ± 3.24pg/ml) levels compared to controls. Findings of indirect hemagglutination assay showed higher antibody titers in the 3.75 mg/kg group (7.200 ± 0.374) compared to higher doses, whereas dose-dependent mortality was observed, with the highest mortality at 15 mg/kg (80%) in the mice lethality assay. (4) Conclusions:…
Read the paper
Related studies
- Structural and topological analysis of thiosemicarbazone-based metal complexes: computational and experimental study of bacterial biofilm inhibition and antioxidant activity
- Toward better cures for Mycobacterium abscessus lung disease
- Challenges for global antibiotic regimen planning and establishing antimicrobial resistance targets: implications for the WHO Essential Medicines List and AWaRe antibiotic book dosing
- Design, Synthesis and In vitro Antitubercular Effect of New Chalcone Derivatives Coupled with 1,2,3‐Triazoles: A Computational Docking Techniques
- Upregulation of pmrA, pmrB, pmrC, phoQ, phoP, and arnT genes contributing to resistance to colistin in superbug Klebsiella pneumoniae isolates from human clinical samples in Tehran, Iran
- Whole-genome sequencing of clinical isolates of Citrobacter Europaeus in China carrying blaOXA−48 and blaNDM−1
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.