Selective six spectrophotometric methods for determination of remdesivir and moxifloxacin hydrochloride for COVID-19 treatment with overlapping spectra: a comprehensive evaluation of greenness, blueness, and whiteness
Abstract
Selective, and green spectrophotometric methods have been developed for the simultaneous analysis of remdesivir (RDV) and moxifloxacin hydrochloride (MFX), two active agents that are being used together in COVID-19 treatment. Because of the considerable spectral overlap, six mathematical spectrophotometric approaches were applied, including ratio derivative, ratio difference, mean centering of ratio spectra, area under the curve, Q-analysis, and bivariate calibration, to allow accurate interference-free determination without preliminary separation. The proposed methods were validated as per ICH guidelines, demonstrating excellent linearity over the concentration ranges of 1–15 µg/mL for RDV and 1–10 µg/mL for MFX, with correlation coefficients exceeding 0.999. They showed high sensitivity with LODs from 0.26 to 0.92 µg/mL and LOQs from 0.27 to 0.96 µg/mL for both drugs, enabling reliable trace analysis of the studied drugs in complex matrices. The methods also applied to dosage forms and spiked human plasma provided good recoveries with minimal matrix interference, demonstrating that the methods are robust and applicable. Eco-Scale, ComplexGAPI, and AGREE gave a high score on green profiles for environmental and practical sustainability. Further, the high whiteness and blueness scores indicated that the methods met the requirements of white analytical chemistry by RGB12 and BAGI tools. Thus, these methods offer a practical, eco-conscious solution for simultaneous…
Read the paper
Related studies
- Structural and topological analysis of thiosemicarbazone-based metal complexes: computational and experimental study of bacterial biofilm inhibition and antioxidant activity
- Toward better cures for Mycobacterium abscessus lung disease
- Challenges for global antibiotic regimen planning and establishing antimicrobial resistance targets: implications for the WHO Essential Medicines List and AWaRe antibiotic book dosing
- Design, Synthesis and In vitro Antitubercular Effect of New Chalcone Derivatives Coupled with 1,2,3‐Triazoles: A Computational Docking Techniques
- Upregulation of pmrA, pmrB, pmrC, phoQ, phoP, and arnT genes contributing to resistance to colistin in superbug Klebsiella pneumoniae isolates from human clinical samples in Tehran, Iran
- Whole-genome sequencing of clinical isolates of Citrobacter Europaeus in China carrying blaOXA−48 and blaNDM−1
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.