Structural characterization, derivatization, and bioactivities of secondary metabolites produced by termite-associated Streptomyces lannensis BYF-106
Abstract
Two new C-glycoside angucycline-related analogs, urdamycin Y (1) and grincamycin W (2), as well as eight known metabolites (3-10), were identified from termite-associated Streptomyces lannensis BYF-106 based on global natural products social molecular networking (GNPS). The putative biosynthetic pathways of urdamycin Y (1) and grincamycin W (2) were proposed using bioinformatic analysis of the full genome of S. lannensis BYF-106. In addition, four new derivative compounds (4A, 5A, 6A, and 6B) were synthesized via acetylation and methylation, respectively. Partial compounds were evaluated in vitro for antibacterial, anti-inflammatory, and cytotoxic activities. Vineomycinone B2 (3), fridamycin D (4), and 6-hydroxytetrangulol (5) displayed broad-spectrum antibacterial activities against S. aureus, methicillin-resistant S. aureus, and P. syringae pv. actinidae. Furthermore, urdamycin Y (1) exhibited potent inhibition on NO production, with an IC50 value of 4.8 µM, which was comparable to that of Bay11-7082 with an IC50 value of 2.1 µM. Subsequently, the possible anti-inflammatory mechanism of urdamycin Y (1) was explored by molecular docking simulation. Finally, most of the tested metabolites showed significant cytotoxic activities against HCT-116, HT-29, and A375. Notably, 6-hydroxytetrangulol (5) and the acetyl derivative 5A showed extremely strong cytotoxic activities against HCT-116, with IC50 values of 9.8 and 2.2 µM, respectively. Moreover, 5A showed extremely strong…
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