Abnormalities in primary cilium of osteoblasts of adolescent idiopathic scoliosis patients
Abstract
Adolescent Idiopathic Scoliosis (AIS) is a common complex genetic disease and one of the most prevalent childhood deformities. AIS is clinically characterized by a 3D spinal deformity with unknown cause. The aim of this study is to identify rare genetic variants and associated biological pathways involved in AIS onset and its progression.
We recently completed exome sequencing of 69 AIS cases vs. 70 matched controls in a French-Canadian AIS cohort using SOLiD 5 technology. Results showed two gene families with significant enrichment for single nucleotide variants (SNVs) involved in cilia signalling pathways. To investigate the functional effect of these SNVs, we explored biomechanical responses on osteoblasts from AIS patients carrying these SNVs. More specifically, we applied a sinusoidal/oscillatory fluid flow at 2Pa, 0.5Hz for 90min using parallel plate flow chamber.
Preliminary qPCR results of the cells post flow showed a significant increase (+10-folds) in the expression of cyclooxygenase-2 (COX2) in AIS cells versus control ones. Interestingly, microscopy imaging of the same cells revealed a 30% increase in the length of cilia (p<0.01) of AIS cells when compared to control ones. Of note, increased COX2 expression has been reported as a flow mediated response for bone cells. Our results suggest an increased sensitivity of AIS bone cells to fluid flow due to the elongation of cilia.
Although cilia abnormalities have never been investigated in idiopathic scoliosis,…
How strong is the evidence?
Evidence Hub reads this as a cohort study, done in people. Its evidence level is 3 of 5 (Moderate · trial / cohort / secondary analysis). Trials without a large randomized design, cohorts and secondary analyses give moderate evidence.
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