Effect of thymoquinone: the extract of nigella sativa in accelerating soft callus formation in fracture
Abstract
Background: Excessive oxidative stress on fracture case can inhibit fracture healing and decrease time of bone healing. Thymoquinone, an active substance of Nigella sativa, the so-called black cumin in common, is a potent antioxidant and have been studied as an antiosteoporotic agent. Thymoquinone is expected to be the adjuvant alternative that enhance the recovery process of fracture cases by reducing oxidative stress and promotes osteoblast proliferation on callus formation.Methods: Among 32 male mice Wistar Strain divided into 2 groups, conducted tibia fracture and casted. Group 1 was the control group without supplementation of Nigella sativa while black-cummin extract were given in group 2 orally at a dose of 800 mg/kg for 14 days. On the 14th day, group 1 and 2 were sacrificed, each bone tissue was taken to measure the levels of MDA by utilizing TBARS method and calculate the number of osteoblasts under the microscope. Data analysis were done using independent t-test.Results: There are both decreased MDA levels and increased number of osteoblasts that are histologically significant to the groups administered by Nigella sativa extract containing Thymoquinone compared to the control groups (p <0.05) on day 14.Conclusions: The administration of Thymoquinone from the extract of Nigella sativa reduced oxidative stress in fractures as well as increase the number of the osteoblast and its differentiation in callus formation.
Read the paper
Related studies
- Irisin Enhances Osteoblast DifferentiationIn Vitro
- Glucocorticoids suppress Wnt16 expression in osteoblasts in vitro and in vivo
- Interaction between Schwann Cells and Osteoblasts In Vitro
- Administration of cinnamaldehyde promotes osteogenesis in ovariectomized rats and differentiation of osteoblast in vitro
- The Effect of the Major Components of Fructus Cnidii on Osteoblasts In Vitro
- Estrogen depletion alters osteogenic differentiation and matrix production by osteoblasts in vitro
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.